The Callisto Sample Prep System represents a breakthrough in genomics laboratory and clinical operations. Composed of a benchtop instrument and associated consumables, it requires no specialized skills or infrastructure to operate, ensuring accessibility for users of all levels. Key features include a diverse range of pre-optimized applications supporting short- and long-read sequencing workflows, flexible batch sizes accommodating up to 24 reactions per run, and enhanced scalability for both low- and high-throughput applications. Additionally, the system significantly reduces hands-on time, plastic waste, and reagent usage, promoting efficiency and sustainability. Leading institutions have already benefited from early access to the system, recognizing its potential to revolutionize sample preparation processes.